The ACTG/IMPAACT Lab Technologist Committee develops and maintains a variety of laboratory SOPs intended for use by laboratories affiliated with the ACTG and IMPAACT networks.
Letter of Introduction
Jan 09, 2009, v1.0, LTC SOP 002
Protocol Participation and Laboratory Processing Chart Preparation Procedures
09Dec2022; v4.0
Lab Processing Chart Template ACTG
27Apr2022
Lab Processing Chart Template IMPAACT
27Apr2022
Division of AIDS (DAIDS) Guidelines for Good Clinical Laboratory Practice (GCLP) Standards
GCLP Online Training
Link to DAIDS Learning Management System - search catalog for "GCLP"
DAIDS Laboratory Audit Process
DAIDS Presentation, 2012
How to Apply for a CLIA Certificate
Centers for Medicare & Medicaid Services informational website
Clinical Pharmacology Quality Assurance (CPQA)
Quality assurance provider for clinical pharmacology
Immunology Quality Assurance (IQA)
Quality assurance provider for flow cytometry and PBMC cryopreservation
Patient Safety in International Laboratories (pSMILE)
Quality assurance provider for patient safety testing
United Kingdom National External Quality Assessment Service (UK NEQAS)
Provider of stabilized samples for flow cytometry proficiency testing at non-US labs
Virology Quality Assurance (VQA)
Quality assurance provider for virology
Biosafety Orientation
See Section I: Introduction of Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition
pSMILE Safety Resources
There are many useful and up-to-date resources for general safety and biosafety on the pSMILE site.
Safety Inspection Checklist for BSL-3 Laboratories
Microbiology laboratory safety checklist developed by the CDC and posted on pSMILE site.
MERIFLUOR® Cryptosporidium/Giardia Assay
Link to SOP on Meridian Bioscience website
Cat. B Shipping SOP
Jun 30, 2016, v3.0 LTC SOP 022
Cat. B Shipment Checklist
Jun 30, 2016 v3.0
Guidelines for Shipment and Receipt of Category A Infectious Specimens and Dangerous Goods
Mar 04, 2008, v1.0, LTC SOP 021
Instructions for Overnight Shipment
May 02, 2008, v2.0, LTC SOP 023
International Shipment Checklist
Sept 01, 2015 version 3
Microscope Slides and Paraffin Block Storage and Handling SOP
February 22, 2018, v1.0, LTC SOP 075
Shipping Addresses and Instructions
Shipping Notice (Ambient, Refrigerated, or Frozen)
April 08, 2015, v4.0
Shipment Evaluation Procedure
May 12, 2022, v8.0
Appendix 1: Shipment Evaluation Form
May 12, 2022 v8.0, LTC SOP 073 Appendix 1 Form
BRI CDC Import Permit
Issued: Aug 24, 2020
NICHD Repository Shipping SOP
June 19, 2020, v5.0
Adipose Tissue Biopsy SOP
Nov 13, 2015, v1.0, LTC SOP 072
Cerebral Spinal Fluid
April 08, 2013, v1.0, LTC SOP 067
Cold Chain Guidelines
Cross-Network Cold Chain Guidelines
Dried Blood Spots (DBS) Processing
Mar 19, 2012, v2.0, LTC SOP 055
Dried PBMC Pellet Processing (LDMS Specimen Code BLD/EDTA or ACD/PEL)
Nov 29, 2012, v.4.0, LTC SOP 059
Female Genital Secretion Collection and Processing
Aug 12, 2015, v2.0, LTC SOP 016
GALT SOP
Apr 21, 2014, v1.0, LTC SOP 069
Leukopak PBMC Processing Standard Operating Procedure
22Nov2022; v2.0; LTC SOP 61
Lumbar Puncture with Sprotte Needle
A video on lumbar puncture using a Sprotte needle, by David B. Clifford, MD.
Lymph Node Biopsy SOP
July 15, 2014, LTC SOP 071
Male Genital Secretion Collection and Processing
Aug 10, 2015, v2.0, LTC SOP 015
Peripheral Blood Mononuclear Cells (PBMC) Cryopreservation
Cross-Network PBMC Processing SOP
PBMC Thawing
Nov 26, 2012, v1.0, LTC SOP 065
Serum Processing from Whole Blood
Feb 05, 2016, v1.0, LTC SOP 066
Umbilical Cord Blood Collection
Jan 18, 2013, v1.0, LTC SOP 063
Collection Storage Transport Expectorated Sputum SOP
July 01, 2014, v1.0, LTC SOP 070
Sputum Processing for Storage
Oct 16, 2015, v1.0 LTC SOP 074
Anemometer Memo
March 09, 2018, TBLDWG memo
Site Tuberculosis Infection Control SOP
March 17, 2017, v1.0, ACTG SOP 160
TB Sourcebook
Mycobacteriology Laboratory Sourcebook for Harmonization and Support of Tuberculosis (TB) Clinical Trials
QFT Plus SOP v1.0
Requirements for collection, processing and storage of specimens for use with the QIAGEN QFT-Plus IGRA
QFT-Plus Blood Collection and Processing Timepoint Collection Form
August 13, 2019
QFT Incubation Time Calculator
Mycobacteriology Laboratory Checklists
March 07, 2018, Checklists from the TB Sourcebook v1.0
Mycobacteriology Laboratory Sourcebook for Harmonization and Support of Tuberculosis (TB) Clinical Trials
v1.0 March 07, 2018, v1.0
Quality Indicator Report Form
March 07, 2018, Sample QI Monthly Report Form
Note: This information applies to ACTG and IMPAACT laboratories only.
Heat-inactivated fetal bovine serum (FBS) is being held on reserve at Sigma Aldrich. Please note that the VQA and IQA validation testing of these lots for use in culture experiments and for Peripheral Blood Mononuclear Cell (PBMC) cryopreservation have been completed. A certificate of analysis of each company's lot is available upon request.
Sigma Aldrich
Batch #:20H250
FBS Heat Inactivated US origin
As of March 3, 2022 a total of 138 (500 mL) of the current batch of heat inactivated FBS remain on reserve with Sigma Aldrich. The price per bottle of $389.00 will be extended through April 30, 2022. This price includes shipping and handling and dry ice fees for a minimum order of 6 bottles (shipping charges will be added for orders less than 6 bottles).
Placing Orders:
Call: 1-800-220-0195
Email: research.teamserum@milliporesigma.com
Fax: 1-800-579-1850
Please have the following information ready when placing your order:
Quote #: 22794126
Material #: F4135-500ml
Batch #: 20H250
Serum Expiration: May, 2025
View the list of ordering contacts for non-U.S. sites
If you have any specific questions relating to this lot of FBS, please contact Sheila Kohlman at Sigma Aldrich at +1-314 -289-6007 / +1-314-873-9935.
FBS Certificate of Analysis LOT 20H250
If you would like a copy of a Certificate of Analysis from a previous lot, please contact Tyler Brown.
Gamma-irradiated Gemini Gamma-irradiated Gemini GemCellTM Bio Products
Lot # A88E82G
FBS Gamma Irradiated Heat-Inactivated US Origin
***Filtration would be recommended after thawing and before aliquoting out.
To order the FBS product, e-mail BARC procurement department:
Attention: Lionel Baeta and May Meyile
Cc: Peter Meewes
Product name and code: BARC Gem cell Bovine Serum, lot number A88E82G
Price per 500/ml bottle: R12455.00
The South African Department of Agriculture now requires that Fetal Bovine Serum (FBS) of foreign origin be gamma irradiated prior to import and use.
The ACTG in collaboration with BARC South Africa identified a lot of gamma-irradiated FBS. The Immunology Quality Assessment Center (IQA) assessed the suitability of the FBS lot for use. The results were acceptable and the ACTG approved the use of this lot of FBS for processing ACTG specimens.
Lot Information:
Catalogue Number: 100-500
Lot Number: A88E82G
Expiration Date: March 2020
FBS Certificate of Analysis
Please verify the Shipping Contact Information and Instructions as indicated on the Protocol LPC and/or lab information on the FSTRF Portal.
Shipping Address
Quest Diagnostics Incorporated
Attn: Special Studies
1715 Twin Springs Road
Baltimore, MD 21227
Phone: 410.314.1551
Fax: 443.833.3862
Email: DGXBaltimoreSpecialStudiesDepartment@questdiagnostics.com
LDMS#: 33
Other Instructions
Sites must register with Quest prior to shipping.
To register, site should contact: Larry Hirsch, 410.314.1547
Ship overnight Monday through Wednesday preferred; Thursday OK.
Mark Thursday shipments as “OK to deliver on Saturday”.
Include appropriate Quest Requisition and LDMS manifest.
Email electronic LDMS manifest to: DGXBaltimoreSpecialStudiesDepartment@questdiagnostics.com
Shipping Address
Biomedical Research Institute
c/o John C. Ward, Jr.
9410 Key West Avenue, First Floor
Rockville, MD 20850
USA
Phone: 301.881.7636
Fax: 301.770.9811
Email: brirepository@afbr-bri.com
LDMS#: 999
Other Instructions
Instructions for shipping ACTG specimens to BRI (ACTG password required)
Instructions for shipping IMPAACT specimens to BRI (IMPAACT password required)
Shipping Address
Fisher Bioservices - NICHD Repository
ATTN: Ryan Patrick Babay
625 Lofstrand Lane
Rockville, MD 20850 USA
Phone: 301.354.0325
Fax: 301.838.9753
Email: fbs.gbo@thermo.com; ryan.busa-babay@thermofisher.com
LDMS#: 243
Other Instructions
For shipping instructions to Fisher BioServices, use the most recent NICHD Repository Shipping SOP found under Shipping Guidelines.