Reagents	Lot Number	Exp. Date
(after opening)	Visual QC:  OK		Batch PBMC Processing and Manual Cell Count Worksheet	
PBS					Process Date:	Process Tech:	
LSM					Freezing Media Prep Time:	Centrifuge Temp:	
FBS					Mr. Frosty/CoolCell #:	Freeze start time:	
DMSO					Transfer from Mr. Frosty/CoolCell:	Date	Time	Tech	
Trypan Blue						Storage: LN2 or -80°C is network/protocol dependent	

PID	LDMS Spec
ID	Anti-
Coagulant	Draw Time	Start Process
Time
(1st cfg)	Start Ficoll Time	Cell Suspension Volume
(for count)
(V)	Total Cell Count      (4 Large squares)
(T)	Dead
Cell Count
(4 Large squares)	Viable Cell 
Count
(4 Large squares)	Percent Viability*	Total # Cells in Suspension 
(x 106)
(C x V)	Final Re-suspension
Volume**
(Vf)	
													
Protocol #:	Visit:		Avg # Cells/square:	Cell counts in range and consistent?  Yes           No	
													
Protocol #:	Visit:		Avg # Cells/square:	Cell counts in range and consistent?  Yes           No	
													
Protocol #:	Visit:		Avg # Cells/square:	Cell counts in range and consistent?  Yes           No	
													
Protocol #:	Visit:		Avg # Cells/square:	Cell counts in range and consistent?  Yes           No	
*  % Viability = (#viable (clear) cells counted/ Total # cells counted)  x 100                                     		**   Vf = (T/N1) x V2         ( N1 = Desired final cell conc.;  V2 = final aliquot volume in mL)	
													
Goal: Draw time to Start Freeze time <8 hours		LDMS Data Entry: Tech/Date			
Required: Start Process time to Start Freeze time <4 hours									
Required: Start Ficoll time to Start Freeze time <3 hours								
Explain any Deviation in Comments.  Indicate if sample collection and process dates are not the same.					
Comments and Protocol Deviations: