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Title

Group M-based HIV-1 Gag peptides are frequently targeted by T cells in chronically infected US and Zambian patients

Authors

A. Bansal, E. Gough, D. Ritter, et al.

Network Affiliation

Other

Organization

 

Journal Name

AIDS

Publication Date

2/14/2006

PubMed Search

http://www.ncbi.nlm.nih.gov/pubmed?term=16439868

Link to full-text

 

PMID

 

Abstract

BACKGROUND:

The enormous sequence diversity of HIV-1 has been a major obstacle in the development of a globally useful vaccine for AIDS. The consensus and ancestral sequence-based immunogens minimize the genetic distance between contemporary isolates and vaccine strains. Hence these sequences may be promising candidates for HIV vaccines or serve as a universal reagent set for evaluating Gag-specific responses.

METHODS:

In this study, we measured the T-cell reactivity to consensus (subtype A, B, C and group M), ancestral (group M and subtype B) and HXB2 Gag peptides (15-mers overlapping by 11) in HIV-1-infected subjects from two reference populations. We evaluated the Gag-specific T-cell responses in 43 chronically infected US (subtype B) and 13 Zambian (subtype C) subjects using an interferon-gamma enzyme-linked immunosorbent spot assay.

RESULTS:

Our findings demonstrate a broad cross-reactivity of nearly 70% among all the seven Gag immunogens evaluated. Consensus M sequences elicited similar levels of responses as did the consensus B, ancestral subtype B and HXB2 peptides in subtype B-infected US patients. In subtype C-infected Zambian subjects, responses of similar breadth and magnitude were elicited by consensus C, consensus M and ancestral M peptides.

CONCLUSION:

Our data demonstrate that peptide pools based on consensus or ancestral M-based sequences can be used to evaluate Gag-specific responses elicited by subtype B or subtype C-based immunogens.

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Topic

 

Attachments

Created at 3/23/2012 10:35 AM by Davis, Gregory P
Last modified at 3/23/2012 10:35 AM by Davis, Gregory P