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ACTG/IMPAACT Laboratory Manual
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In addition to the documents provided below, ACTG/IMPAACT laboratories should follow the guidelines in:

 

 

 

 Introduction

 
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Letter of Introduction.pdfLetter of Introduction
 

 Laboratory Guidelines

 
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ACTG GLP Checklist.pdfACTG GLP Checklist
Biosafety Checklist.pdfBiosafety Checklist
Biosafety Orientation.pdfBiosafety Orientation
Certification Requirements for Laboratories Participating in ACTG Studies.pdfCertification Requirements for Laboratories Participating in ACTG Studies
Good Laboratory Practices (GLP) Document.pdfGood Laboratory Practices (GLP) Document
 

 Specimen Processing Methods

 
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ACTG Central Metabolic Laboratory Specimen Processing.pdfACTG Central Metabolic Laboratory Specimen Processing
Dried Blood Spots Card Collection, Processing and Storage Procedures.pdfDried Blood Spots Card Collection, Processing and Storage Procedures
Female Genital Secretion Collection and Processing.pdfFemale Genital Secretion Collection and Processing
Male Genital Secretion Collection and Processing.pdfMale Genital Secretion Collection and Processing
Pediatric Stool Processing for Ova and Parasites.pdfPediatric Stool Processing for Ova and Parasites
Procedure for Excisional Lymph Node Biopsy.pdfProcedure for Excisional Lymph Node Biopsy
Standard Plasma Pharmacology Sampling.pdfStandard Plasma Pharmacology Sampling
 

 Shipping Guidelines

 
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ACTN Ambient Specimen.docACTN Ambient Specimen
ACTN Frozen Specimen.docACTN Frozen Specimen
ACTN Guidelines for Shipment and Receipt of Category A Infectious Specimens and Dangerous Goods.pdfACTN Guidelines for Shipment and Receipt of Category A Infectious Specimens and Dangerous Goods
ACTN Guidelines for Shipment and Receipt of Category B Biological Substance Shipment.pdfACTN Guidelines for Shipment and Receipt of Category B Biological Substance Shipment
ACTN Instructions for Overnight Shipment.pdfACTN Instructions for Overnight Shipment
 

 Immunologic Methods

 
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ACTN Consensus Method for 3-color Advanced Flow.pdfACTN Consensus Method for 3-color Advanced Flow
Apoptosis- PACTG TUNEL Method.pdfApoptosis- PACTG TUNEL Method
Apoptosis- PI Method.pdfApoptosis- PI Method
Dye Dilution (CFSE) Proliferation.pdfDye Dilution (CFSE) Proliferation
Lymphocyte Proliferation Assay.pdfLymphocyte Proliferation Assay
Sample Analysis of Histogram (3-color).pdfSample Analysis of Histogram (3-color)
 

 Virologic Methods

 
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Bayer TruGene HIV-1 Genotyping.pdfBayer TruGene HIV-1 Genotyping
Celera ViroSeq HIV-1 Genotyping.pdfCelera ViroSeq HIV-1 Genotyping
HIV Qualitative CSF-PBMC.pdfHIV Qualitative CSF-PBMC
HIV Qualitative PBMC Macrococulture.pdfHIV Qualitative PBMC Macrococulture
HIV Qualitative PBMC Micrococulture.pdfHIV Qualitative PBMC Micrococulture
Perkin-Elmer (NEN Life Sciences) Hiv-1 p24 Antigen ELISA.pdfPerkin-Elmer (NEN Life Sciences) Hiv-1 p24 Antigen ELISA
Preparation of PHA-Stimulated Uninfected Donor PBMCs.pdfPreparation of PHA-Stimulated Uninfected Donor PBMCs
Roche Amplicor HIV-1 DNA Test, v1.0.pdfRoche Amplicor HIV-1 DNA Test, v1.0
Standard Roche Monitor Test, Boom Extraction.pdfStandard Roche Monitor Test, Boom Extraction
Standard Roche Monitor Test, v1.5, Microwell Plate.pdfStandard Roche Monitor Test, v1.5, Microwell Plate
UltraSensitive Roche Monitor Test, Boom Extraction.pdfUltraSensitive Roche Monitor Test, Boom Extraction
UltraSensitive Roche Monitor Test, v1.5, Microwell Plate.pdfUltraSensitive Roche Monitor Test, v1.5, Microwell Plate
 

 Immunologic Methods Archive

 
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CD38 Quantitation Assay.pdfCD38 Quantitation Assay
Cytotoxic T-Lymphocyte Method 1.pdfCytotoxic T-Lymphocyte Method 1
Cytotoxic T-Lymphocyte Method 2.pdfCytotoxic T-Lymphocyte Method 2
Inducible Cytokine Whole Blood Method.pdfInducible Cytokine Whole Blood Method
Ki-67 Proliferation.pdfKi-67 Proliferation
 

 Virologic Methods Archive

 
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NameUse SHIFT+ENTER to open the menu (new window).
  
Beckman-Coulter HIV-1 p24 Antigen ELISA.pdfBeckman-Coulter HIV-1 p24 Antigen ELISA
Biomerieux NucliSens HIV-1 QT.pdfBiomerieux NucliSens HIV-1 QT
Ghost Cell Assay.pdfGhost Cell Assay
HIV Quantitative PBMC Micrococulture.pdfHIV Quantitative PBMC Micrococulture
HIV Syncytium-Inducing (MT-2) Assay.pdfHIV Syncytium-Inducing (MT-2) Assay
TCID50 Determination.pdfTCID50 Determination
Zeptometrix RETRO-TEK HIV-1 p24 Antigen ELISA.pdfZeptometrix RETRO-TEK HIV-1 p24 Antigen ELISA
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