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ACTG/IMPAACT Lab Manual

Many of the documents available for download as part of the ACTG/IMPAACT Lab Manual are pdf files that require Adobe Reader. Click here to download the most recent version of Adobe Reader for free.

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Laboratory Guidelines
06-ALM-Biosafety-Orientaion.pdfBiosafety Orientation
07-ALM-Biosafety-Checklist.pdfBiosafety Checklist
08-ALM-Good-Lab-Practices.pdfGood Laboratory Practices (GLP) Document
09-ALM-Good-Lab-Practices-Checklist.pdfACTG GLP Checklist
10-ALM-Certification-Requirements-for-Labs-in-ACTG.pdfCertification Requirements for Laboratories Participating in ACTG Studies

 

Specimen Processing Methods
14-ALM-Procedure-for-Excisional-Lymph-Node-Biopsy.pdfProcedure for Excisional Lymph Node Biopsy
15-ALM-Male-Genital-Secretion-Collection-Processing .pdfMale Genital Secretion Collection and Processing
16-ALM-Female-Genital-Secretions-Collection-Processing.pdfFemale Genital Secretion Collection and Processing
17-ALM-Stool-Processing-for-ova-and-Parasites.pdfPediatric Stool Processing for Ova and Parasites
18-ALM-ACTG-Central-Metabolic-Processing-Guidelines.pdfACTG Central Metabolic Laboratory Specimen Processing
19-ALM-Standard-PLASMA-Pharmacology-Sampling.pdfStandard Plasma Pharmacology Sampling
55-ALM-Dried-Blood-Spots-SOP.pdfDried Blood Spots Card Collection, Processing and Storage Procedures

 

Shipping Guidelines
21-ALM-Guidelines-Ship-CatA.pdfACTN Guidelines for Shipment and Receipt of Category A Infectious Specimens and Dangerous Goods
22-ALM-Guidelines-Ship-CatB.pdfACTN Guidelines for Shipment and Receipt of Category B Biological Substance Shipment
23-ALM-Instructions-for-Overnight-Shipments-revised-10-2007.pdfACTN Instructions for Overnight Shipment
56-ALM-Ambient-Specimen-Shipment-Notice.docACTN Ambient Specimen Shipment Notice, Dated 06/04/07
57-ALM-Frozen-Specimen-Shipment-Notice.docACTN Frozen Specimen Shipment Notice - Dated 06/04/07

 

Immunologic Methods
24-ALM-Consensus-Method-for-3-color-Advanced-Flow.pdfACTN Consensus Method for 3-color Advanced Flow
25-ALM-3-Color-Adv-Flow-Appendix-1.pdfSample Analysis of Histogram (3-color)
27-ALM-Apoptosis-PI-Method.pdfApoptosis- PI Method
28-ALM-Pediatric-TUNEL-Method.pdfApoptosis- PACTG TUNEL Method
29-ALM-Lymphocyte-Proliferation-Assay.pdfLymphocyte Proliferation Assay
30-ALM-Dye-Dilution-Assay-CFSE.pdfDye Dilution (CFSE) Proliferation

 

Virologic Methods
36-ALM-Preparation-of-PHA-Stimulated-Uninfected-Donor-PBMC.pdfPreparation of PHA-Stimulated Uninfected Donor PBMCs
38-ALM-HIV-Qual-Microculture.pdfHIV Qualitative PBMC Micrococulture
39-ALM-HIV-Qualitative-Macrococulture.pdfHIV Qualitative PBMC Macrococulture
40-ALM-HIV-Qualitative-CSF-PBMC-Microculture-Assay.pdfHIV Qualitative CSF/PBMC Micrococulture
45-ALM-Perkin-Elmer-HIV-1-p24-ELISA.pdfPerkin-Elmer (NEN Life Sciences) Hiv-1 p24 Antigen ELISA
47-ALM-Roche-Amplicor-HIV-1-DNA-Test.pdfRoche Amplicor HIV-1 DNA Test, v1.0
48-ALM-Standard-Roche-Monitor-Test-MWP.pdfStandard Roche Monitor Test, v1.5, Microwell Plate
49-ALM-Standard-Roche-Monitor-Test-Boom-Extraction.pdfStandard Roche Monitor Test, Boom Extraction
50-ALM-UltraSensitive-Roche-Monitor-Test-MWP.pdfUltraSensitive Roche Monitor Test, v1.5, Microwell Plate
51-ALM-UltraSensitive-Roche-Monitor-Test-Boom-Extract.pdfUltraSensitive Roche Monitor Test, Boom Extraction
53-ALM-Celera-ViroSeq-HIV-1-Genotyping.pdfCelera ViroSeq HIV-1 Genotyping
54-ALM-Bayer-HIV-1-TRUGENE-Genotyping-Method.pdfBayer TruGene HIV-1 Genotyping

 

Immunologic Methods Archive

For ELISA, see manufacturer's package insert.
26-ALM-CD38-Quantitation-Assay.pdfCD38 Quantitation Assay
31-ALM-Intracellular-Ki67-Staining.pdfKi-67 Proliferation
32-ALM-CTL-Method-1.pdfCytotoxic T-Lymphocyte Method 1
33-ALM-CTL-Method-2.pdfCytotoxic T-Lymphocyte Method 2
34-ALM-Inducible-Cytokine-Whole-Blood-Method.pdfInducible Cytokine Whole Blood Method

 

Virologic Methods Archive
37-ALM-HIV-Quant-PBMC-Culture.pdfHIV Quantitative PBMC Micrococulture
41-ALM-HIV-syncytium-inducing-MT2-assay.pdfHIV Syncytium-Inducing (MT-2) Assay
42-ALM-Ghost-Cell-Assay.pdfGhost Cell Assay
43-ALM-TCID50-Determination.pdfTCID50 Determination
44-ALM-Coulter-HIV1-p24-ELISA.pdfBeckman-Coulter HIV-1 p24 Antigen ELISA
46-ALM-Zeptometrix-RETRO-TEK-HIV1-p24-ELISA.pdfZeptometrix RETRO-TEK HIV-1 p24 Antigen ELISA
52-ALM-BioMerieux-NucliSens-HIV-1-QT.pdfBiomerieux NucliSens HIV-1 QT
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